Thermo Fisher Scientific NUMB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.5 µg/mL

-

Immunohistochemistry (IHC)

5 µg/mL

-

Immunocytochemistry (ICC/IF)

5-20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 17 amino acid peptide near the carboxy terminus of human NUMB. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NUMB,uniProtId:P49757-1,ncbiNodeId:9606,antigenRange:651,antigenLength:651,antigenImageFileName:PA5-21143_NUMB_P49757-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21143_NUMB_P49757-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154464

Product Specific Information

A suggested positive control is mouse lung tissue lysate.

PA5-21143 can be used with blocking peptide PEP-1257.

Target Information

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.