Thermo Fisher Scientific PDIA6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:250

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Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(476) I D L S D V E L D D L G K D E L(492) of human P5.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2161009

Product Specific Information

PA3-008 detects P5 protein in human samples.

PA3-008 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing P5 in human fibrosarcoma HT 1080 cell lysate.

The PA3-008 immunogen is a synthetic peptide corresponding to residues D(476) I D L S D V E L D D L G K D E L(492) of human P5. This peptide (Cat. # PEP-224) is available for use in neutralization and control experiments.

Target Information

P5 is a novel protein that was originally identified in humans. Functionally, P5 has been shown to have peptide binding ability and chaperone activity specific to certain proteins. P5 shares several structural similarities to PDI, an important protein of the ER. The deduced amino acid sequence of P5 shows that it contains the ER retention signal KDEL. P5 also contains two CXXC-like motifs, a motif responsible for oxidoreductase activity. These two CXXC motifs are found on the N- and C-terminus of the protein, one at each end. Studies suggest that the N-terminal CXXC motif is more important than the second for isomerase activity. Contrary to most of the PDI-like proteins, P5 is not stress-inducible.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.