Thermo Fisher Scientific GPR18 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:3000

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Product Specifications

Species Reactivity

Human

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2540485

Product Specific Information

IHC (P) analysis shows positive staining of GPR18 in human enteric ganglion tissue.

WB analysis shows GPR18 in glycoprotein-enriched fractions from GPR18 overexpressing 293 cells. A band of similar MW was also detected in GPR3 overexpressing 293 cells, suggesting some cross-reactivity with GPR3 or enhanced detection of basal GPR18 in the enriched fractions.

While GPR18 has a theoretical MW of ~ 38kD, the observed band in a WB runs at ~70kD due to enrichment of the glycosylated receptor.

Target Information

The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. GPR18 is an orphan G protein-coupled receptor found at high levels in the testis, small intestines and cells associated with the immune system including lymphocytes, thymus and spleen. GPR18 has been found to be a receptor for endogenous lipid neurotransmitters, several of which also bind to cannabinoid receptors. Some ligands described to be GPR18 agonists or partial agonists include N-Arachidonoylglycine, Abnormal cannabidiol, O-1602, AM-251, Cannabidiol, delta 9-Tetrahydrocannabinol, N-arachidonoyl ethanolamine and Arachidonylcyclopropylamide. Antagonists include the synthetic cannabidiol related compounds CID-85469571 and O-1918.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.