Thermo Fisher Scientific HSP90 beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

10-20 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

1-20 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP90 beta,uniProtId:P11499-1,ncbiNodeId:10090,antigenRange:3-13,antigenLength:724,antigenImageFileName:PA3-012_HSP90_beta_P11499-1_Rabbit.svg,antigenImageFileNamePDP:PA3-012_HSP90_beta_P11499-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2121220

Product Specific Information

PA3-012 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86.

PA3-012 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~84 kDa protein representing HSP84 from Hepa1 cell lysate. Immunofluorescence staining of HSP84 in Hepa1 cells with PA3-012 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

The PA3-012 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.