Thermo Fisher Scientific Smoothelin Monoclonal Antibody (R4A)
Smoothelin 단백질을 검출하는 R4A 클론 단일클론 항체로, 사람 및 다양한 종에서 반응. Western blot, IHC, ICC/IF에 적합하며, 분화된 평활근세포 식별에 유용. 액상 형태로 1 mg/mL 농도, 장기보관 시 -20°C 권장.
- 카탈로그번호
- OMA106020
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific Smoothelin Monoclonal Antibody (R4A)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Cat, Human, Mouse, Non-human primate, Pig |
| Published Species | Human, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R4A |
| Immunogen | Cytoskeletal smoothelin extract of chicken gizzard |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2239715 |
Product Specific Information
OMA1-06020 detects smoothelin from human, canine, feline, porcine, chicken, and monkey tissues. This antibody recognizes both visceral and vascular smoothelin isoforms and does not cross-react with rat samples.
It has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures.
By Western blot, this antibody detects ~59 kDa (visceral smoothelin) and ~100 kDa (vascular smoothelin) proteins from canine smooth muscle extracts.
Useful for discriminating fully differentiated (contractile) smooth muscle cells from proliferative ones.
For formalin-fixed paraffin-embedded tissues, boiling in 10 mM citrate buffer (pH 6.0) for 3×5 min prior to immunostaining is recommended.
Antigen: cytoskeletal smoothelin extract of chicken gizzard.
Store at 4ºC, or in small aliquots at -20ºC.
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton and is exclusively expressed in fully differentiated (contractile) SMCs.
Antibodies against smoothelin are useful tools to monitor SMC differentiation.
RNA and protein analyses show broad species distribution.
Cells with SMC-like characteristics (e.g., myofibroblasts, myoepithelial, skeletal, and cardiac muscle cells) do not contain smoothelin.
Confocal microscopy and transfection studies demonstrate a filamentous organization distinct from desmin and vimentin, with smoothelin colocalizing with actin stress fibers.
Two isoforms have been identified:
- 59 kDa (visceral SMC)
- 100 kDa (vascular SMC)
Human smoothelin is encoded by a single-copy gene located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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