Thermo Fisher Scientific Cardiotin Monoclonal Antibody (R2G)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:100

-

Product Specifications

Species Reactivity

Dog, Chicken, Cat, Goat, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat, Xenopus, Zebrafish

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

R2G

Immunogen

Total protein extract of chicken gizzard.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325532

Product Specific Information

OMA1-06030 detects cardiotin from mouse, rat, hamster, canine, monkey, pig, cat, rabbit, frog, goat, xenopus,feline and human cardiomyocytes.

OMA1-06030 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~300 kDa protein representing the cardiotin protein complex, as well as ~100 kDa and ~60 kDa bands representing the cardiotin subunits from rat heart extract.

The OMA1-06030 immunogen is total protein extract from chicken gizzard.

Target Information

Cardiotin is a recently discovered, high molecular weight protein complex (~300 kDa) located in the longitudinal SR of cardiomyocytes. The cardiotin complex exists as 60 kDa and 100 kDa subunits. Both subunits contain the same amino-terminal 14 amino-acid sequence, and show high homology to human skeletal muscle alpha-actin. The tetrameric configuration of the cardiotin protein complex suggests that it exists as a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. Immunofluorescence studies colocalize cardiotin with SERCA 2a ATPase in the longitudinal sarcoplasmic reticulum (SR), although cardiotin is not expressed in junctional SR with SERCA 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.