Thermo Fisher Scientific Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

ELISA (ELISA)

10 µg/mL

View 6 publications 6 publications

Immunoprecipitation (IP)

25 µg/mL*

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

mAbP2-1

Immunogen

Native, secreted form of APP if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Amyloid,uniProtId:P05067-1,ncbiNodeId:9606,antigenRange:1-770,antigenLength:770,antigenImageFileName:OMA1-03132_beta_Amyloid_P05067-1_House_mouse.svg,antigenImageFileNamePDP:OMA1-03132_beta_Amyloid_P05067-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_325526

Product Specific Information

OMA1-03132 detects amyloid precursor protein (APP) from human and monkey tissues. This antibody is specific for native, non-denatured protein, and does not cross-react with mouse, rat APP or other APP homologs.

OMA1-03132 has been successfully used in Western blot (non-reducing conditions), immunocytochemistry, immunoprecipitation and ELISA procedures. This antibody does not work in in IHC on paraffin embedded tissues nor does it recognize APP if the samples are boiled in Laemmli (SDS) sample buffer containing a reducing agent such as DTT or ß-ME. Can be used in immunoprecipitation utilizing 25 µg/mL of OMA1-03132 linked sepharose.

OMA1-03132 antigen is the native, secreted form of human APP. The epitope recognized by OMA1-03132 maps to residues 104-118 of APP

Target Information

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimers disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down`s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.