Thermo Fisher Scientific Vimentin Monoclonal Antibody (RV202)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:200

View 7 publications 7 publications

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish

Published species

Human, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RV202

Immunogen

Cytoskeletal vimentin extract of calf lens. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vimentin,uniProtId:P48616-1,ncbiNodeId:9913,antigenRange:1-466|1-408,antigenLength:466,antigenImageFileName:OMA1-06001_Vimentin_P48616-1_House_mouse.svg,antigenImageFileNamePDP:OMA1-06001_Vimentin_P48616-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325529

Product Specific Information

OMA1-06001 detects vimentin from mouse, rat, hamster, chicken, goat, canine, monkey, zebrafish, porcine and human tissues and cells.

OMA1-06001 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects a ~57 kDa protein representing vimentin from human mesenchymal cells.

OMA1-06001 antigen is cytoskeletal vimentin extract of calf lens.

Store at 4ºC or at -20ºC if preferred. Avoid freeze-thaw cycles.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.