Takara Xfect Transfection Reagent

An obvious way to increase transfection efficiency is to increase the amount of plasmid DNA and transfection reagent mixture that you add to your cells. Unfortunately, since most transfection reagents damage cells during transfection, simply using more of it is not an option. Xfect is different, it allows you to increase your transfection efficiency to the level you need...without killing your cells.

An obvious way to increase transfection efficiency is to increase the amount of plasmid DNA and transfection reagent mixture that you add to your cells. Unfortunately, since most transfection reagents damage cells during transfection, simply using more of it is not an option. The Xfect reagent is different—it allows you to increase your transfection efficiency to the level you need, without killing your cells.

The Xfect reagent is a biodegradable transfection polymer, screened from a pool of 2,300 candidates for its very low cytotoxicity profile and high transfection efficiency. The protocol is far simpler than with most commercially available transfection reagents, it is performed in a single tube, in the presence of serum, and with no requirement for optimization of plasmid to polymer ratios.

The Xfect reagent also outperforms popular competitor transfection reagents in Jurkat cells, which are notoriously difficult to transfect. Xfect Transfection Reagent produced >40-fold higher transfection efficiency than Product L. This transfection reagent can also be used to transfect human adult stem cells. We used the Xfect reagent to transfect human adipose-derived stem cells (hADSCs) with a transfection efficiency of 92%.

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Overview

Excellent for most cell types, even difficult-to-transfect cells

Xfect transfects the five most commonly transfected cell lines with very high efficiency:

• High transfection efficiency and very low cytotoxicity
• Transfect a broad range of cell types
• Convenient split packaging (2 x 50 rxns, or 3 x 100 rxns); share a kit with your colleagues
• Simple, single-tube, serum-compatible protocol
• No Opti-MEM needed, everything is included
• No optimization of ratios required
• 1 reaction corresponds to a transfection in a well of a 6-well plate

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Applications

• Transfection reagent for most commmonly used cell lines

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.