Thermo Fisher Scientific NOV Polyclonal Antibody, Biotin, PeproTech

Applications

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:

MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human NOV. Anti-Human NOV-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hNOV by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NOV (500-P257) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hNOV.

Western Blot:
To detect hNOV by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hNOV is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P257BT-1MG will be provided as 2 × 500 µg.

Target Information

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation correlate with decreased NOV expression. Additionally, NOV induces cell adhesion and migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and fibulin 1C. NOV has also been reported to exert proangiogenic activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.