Thermo Fisher Scientific LIMP2 Polyclonal Antibody
Thermo Fisher Scientific의 LIMP2 Polyclonal Antibody는 다양한 종에 반응하는 Rabbit IgG 기반 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다중 응용에 적합합니다. HDL 수용체 연구 및 SR-BI/SR-BII 관련 콜레스테롤 대사 연구에 활용됩니다.
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Thermo Fisher Scientific LIMP2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Hamster, Human, Mink, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide containing residues from mouse SR-BII (400–478) plus N-terminal cysteine, coupled to KLH [UniProt# O35114] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.1% sodium azide |
| Storage Conditions | −20 °C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2238767 |
Target Information
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism, with plasma concentrations inversely correlated with atherosclerosis risk. SR-BI and SR-BII (also known as SR-BI.2) are splice variants of a single gene. SR-BII differs from SR-BI in its c-terminal cytoplasmic domain and binds HDLs to mediate selective uptake of HDL cholesteryl ester, though with approximately four-fold lower efficiency than SR-BI.
SR-BII is abundant in mouse tissues expressing SR-BI, including liver, adrenal glands, and testes. Although its role remains partially unclear, SR-BII is suggested to function as an HDL receptor. SR-BII mRNA results from alternative splicing of SR-BI precursor transcripts, with the SR-BII isoform mediating selective lipid transfer between HDL and cells. The differential expression and activity of SR-BI and SR-BII may regulate lipid exchange between HDL and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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