Thermo Fisher Scientific LIMP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:50-1:250

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LIMP2,uniProtId:O35114-1,ncbiNodeId:10090,antigenRange:478,antigenLength:478,antigenImageFileName:PA3-16802_LIMP2_O35114-1_Rabbit.svg,antigenImageFileNamePDP:PA3-16802_LIMP2_O35114-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2182836

Product Specific Information

Suggested positive control: 3T3 whole cell lysate.

Target Information

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. Although the role of SR-BII is not completely clear, research suggests that it may be a functional HDL receptor. In addition, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.