
Thermo Fisher Scientific EIF4E Monoclonal Antibody (OTI5D11), TrueMAB
EIF4E 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC(P), Flow cytometry에 사용 가능. 고순도 친화 크로마토그래피 정제 및 동결건조 형태로 제공되며, 인체 시료에 반응. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D11 |
| Immunogen | Full length human recombinant protein of human EIF4E produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
eIF4E participates in various cellular processes such as translational efficiency, splicing, mRNA stability, and RNA nuclear export.
All eukaryotic mRNAs are capped at their 5′ ends with the 7-methylguanosine structure (m7GpppX).
EIF4E is a eukaryotic translation initiation factor directing ribosomes to the cap structure of mRNAs.
It exists as a 24-kDa polypeptide in free form or as part of the EIF4F complex.
EIF4E is the rate-limiting component in the mRNA-ribosome binding step of protein synthesis.
Other EIF4F subunits include EIF4A (50 kDa, ATPase/RNA helicase) and EIF4G (220 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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