Thermo Fisher Scientific HLA-A2 Monoclonal Antibody (BB7.2)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1 µg/mL
• View 1 publication

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent
• View publication

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 647
• PE
• Request custom conjugation

Product Specific Information

This antibody recognizes an extracellular epitope at the C-terminus of the alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen.

Target Information

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.

The heavy chain (~45 kDa) gene contains 8 exons:

1. Exon 1: leader peptide
2. Exons 2–3: alpha1 and alpha2 domains (peptide binding)
3. Exon 4: alpha3 domain
4. Exon 5: transmembrane region
5. Exons 6–7: cytoplasmic tail

Polymorphisms within exon 2 and exon 3 determine peptide binding specificity of each class I molecule. Typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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