Thermo Fisher Scientific Phospho-Mer2 (Ser30) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

ELISA (ELISA)

1:5,000-1:25,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated peptide to remove any unwanted reactivities.

Target Information

Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.