Thermo Fisher Scientific MCM2 Polyclonal Antibody
Thermo Fisher Scientific의 MCM2 Polyclonal Antibody는 인간, 마우스, 랫, 효모에 반응하는 친화 정제된 항체입니다. Western blot과 ELISA에 적합하며, N-말단 영역(aa 15–40)에 대한 면역원으로 제작되었습니다. DNA 복제 관련 MCM2 단백질 연구용으로 안정적인 액상 형태로 제공됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1:10,000–1:50,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminal region near amino acids 15–40 of human MCM2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.98 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against human MCM2 protein. It shows equivalent reactivity against both unphosphorylated and phosphorylated forms (phosphorylated at residues pS26 and pS27).
BLAST analysis suggests cross-reactivity with MCM2 proteins from human, mouse, rat, and S. cerevisiae based on 100% homology with the immunizing sequence.
Reactivity against homologues from other sources is not known.
Target Information
MCM2 (Mini chromosome maintenance protein 2) regulates DNA replication and is crucial for the cell cycle, particularly in the onset of DNA replication and cell division.
Also known as CDCL1, mitotin, and BM28, MCM2 is a nuclear protein mapped to chromosome 3q21.
CDCL1 is considered a candidate oncogene associated with chromosomal breaks in acute myeloid leukemia (AML).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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