Thermo Fisher Scientific XBP1 Polyclonal Antibody
XBP1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 인간 및 생쥐 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 2–8°C 보관, 장기 보관 시 -20°C 권장.
- 카탈로그번호
- PA520218
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–5 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 17 amino acids near the amino terminus of human XBP-1 (amino acid range: 40–90) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months; for long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153798 |
Product Specific Information
- Suggested positive control: HepG2 cell lysate
- PA5-20218 can be used with blocking peptide PEP-0351
Target Information
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and generates isoform XBP1(S), the functionally active transcription factor. The unspliced isoform XBP1(U) is constitutively expressed and acts as a negative feedback regulator of XBP1(S), shutting off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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