Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor 660, eBioscience
E-Cadherin(CD324)을 인식하는 DECMA-1 단클론 항체로, eFluor 660 형광 표지. 인간, 마우스, 개 반응성. 세포 접착 및 암 연구에 적합. Flow Cytometry, ICC/IF 등 다양한 응용 가능.
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Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor 660, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 5 |
| Immunohistochemistry (IHC) | - | 8 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | 11 |
| Flow Cytometry (Flow) | 0.5 µg/test | 15 |
| Neutralization (Neu) | - | 3 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DECMA-1 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11043137 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- Functional Grade
- PerCP-eFluor 710
- Custom conjugation available
Product Specific Information
The monoclonal antibody DECMA-1 recognizes mouse, human, and canine CD324 (E-cadherin or uvomorulin). E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion, sharing structural features with P- and N-cadherin. Its extracellular domains contain Ca²⁺-binding motifs, while the cytoplasmic domain interacts with catenins and actinins, linking to the cytoskeleton.
E-cadherin is expressed in most epidermal cells and localized at intercellular boundaries of epithelial tissues, maintaining tissue integrity. Loss of E-cadherin function is associated with cancer progression. The DECMA-1 antibody disrupts adhesion in human, mouse, and dog cells.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometry of MDCK cells; optimal staining achieved by intracellular staining. Recommended ≤0.5 µg/test (100 µL final volume).
Note: Avoid trypsin exposure; E-cadherin is sensitive to trypsin.
Filtration: 0.2 µm post-manufacturing filtered.
eFluor™ 660 replaces Alexa Fluor™ 647 (Excitation: 633–647 nm; Emission: 668 nm; Laser: Red).
Target Information
E-Cadherin (epithelial cadherin) is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. Mutations in CDH1 (chromosome 16) are linked to gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-cadherin contributes to increased proliferation, invasion, and metastasis. It also mediates bacterial adhesion and internalization. E-cadherin is essential for tissue architecture and integrity.
For Research Use Only. Not for use in diagnostic procedures.
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