Thermo Fisher Scientific Kindlin-1/KIND1 Monoclonal Antibody (4A5.14)
Kindlin-1 단백질을 인식하는 Mouse 모노클로날 항체로, WB, IHC(P), IP 등에 사용 가능. Human 시료 반응성, 비결합형 액상 형태이며 Protein A/G로 정제됨. 세포 부착 및 integrin 신호 연구에 적합.
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Thermo Fisher Scientific Kindlin-1/KIND1 Monoclonal Antibody (4A5.14)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunoprecipitation (IP) | 1–2 µg per 100–500 µg of protein |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4A5.14 |
| Immunogen | Recombinant protein containing Kindlin-1 (residues 216–677) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Kindlin-1 is a member of the kindlin family of focal adhesion proteins containing a FERM domain and a pleckstrin homology domain. It is involved in:
- Integrin signaling and cytoskeleton–extracellular matrix linkage
- Cell adhesion and keratinocyte proliferation
- Activation of ITGA2B when coexpressed with talin
Mutations in Kindlin-1 are associated with Kindler syndrome, a recessive skin disorder characterized by blistering, photosensitivity, poikiloderma, and atrophy.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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