Thermo Fisher Scientific Kindlin-1/KIND1 Monoclonal Antibody (4A5.14)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunoprecipitation (IP)

1-2 µg/100-500 µg of protein

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, lambda

Class

Monoclonal

Type

Antibody

Clone

4A5.14

Immunogen

Recombinant protein containing Kindlin-1 (residues 216-677) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kindlin,uniProtId:Q9BQL6-1,ncbiNodeId:9606,antigenRange:216-677,antigenLength:677,antigenImageFileName:55612-MSM1-P1_Kindlin_Q9BQL6-1_House_mouse.svg,antigenImageFileNamePDP:55612-MSM1-P1_Kindlin_Q9BQL6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Kindlin 1 is a member of the kindlin family of focal adhesion proteins and contains a FERM domain and pleckstrin homology domain. It is involved in integrin signaling, linkage of the cytoskeleton to the extracellular matrix, and cell adhesion. It is most often expressed in epithelial cells. When kindlin 1 is coexpressed with talin it helps to activate ITGA2B. It is required for keratinocyte proliferation, polarization of basal keratinocytes in the skin, and for normal cell shape. Mutations in kindlin 1 have been linked to Kindler syndrome, a recessive skin disorder involving blistering, photosensitivity, poikiloderma, and atrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.