Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Recombinant Rabbit Monoclonal Antibody (VIM/6576R)
Vimentin 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, 중간엽 세포 마커 연구에 적합합니다. Western blot 및 면역조직화학(IHC) 분석에 사용 가능하며, 다양한 종에 반응합니다. 단백질 A/G로 정제된 고순도 항체로 4°C에서 안정적으로 보관됩니다.
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Thermo Fisher Scientific Vimentin (Mesenchymal Cell Marker) Recombinant Rabbit Monoclonal Antibody (VIM/6576R)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Cat, Human, Pig, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIM/6576R |
| Immunogen | Recombinant full-length human Vimentin protein (UniProt ID: P08670-1, amino acids 1–466) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early stages of cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by mediating cholesterol transport within cells. Phosphorylation of vimentin leads to cytoskeletal reorganization facilitating cholesterol transfer to mitochondria, thereby enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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