Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 6430R)

Applications

Western Blot (WB)

• Tested Dilution: 2–4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• For formalin-fixed tissues: heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at room temperature for 20 min.
• This antibody shows broad species reactivity and does not react with Mouse.

Target Information

Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific intermediate filament proteins. It is implicated in steroid synthesis regulation by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin triggers cytoskeletal rearrangement facilitating cholesterol transport to mitochondria, enhancing steroid synthesis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.