Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 6430R)
Vimentin 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체로, Western blot과 IHC에 최적화됨. 다양한 종에 반응하며 Mouse에는 반응하지 않음. 고순도 Protein A/G 정제, PBS buffer에 보존, 연구용 전용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Cat, Human, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIM, 6430R |
| Immunogen | Recombinant fragment (aa 2–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
- Broad species reactivity; does not react with Mouse.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport between the nucleus and plasma membrane. Unlike other IFPs, vimentin is expressed along with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to intermediate filament breakdown and cytoskeletal changes facilitating cholesterol transport to mitochondria, increasing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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