Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 4388R)

Applications

Western Blot (WB)

• Assay-dependent

Immunohistochemistry (Paraffin) (IHC-P)

• 1–2 µg/mL

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. During development, vimentin is expressed along with desmin and later replaced by tissue-specific IFPs.
Vimentin also plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.