Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 4388R)
Recombinant rabbit monoclonal antibody targeting human Vimentin (aa366–466). Validated for WB and IHC-P applications. High purity via Protein A/G purification. Supplied as 200 µg/mL liquid in PBS buffer with BSA and sodium azide. For research use only.
- 카탈로그번호
- 7431-RBM7-P0
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Vimentin Recombinant Rabbit Monoclonal Antibody (VIM, 4388R)
Applications
Western Blot (WB)
- Assay-dependent
Immunohistochemistry (Paraffin) (IHC-P)
- 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VIM, 4388R |
| Immunogen | Recombinant fragment (around aa366–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. During development, vimentin is expressed along with desmin and later replaced by tissue-specific IFPs.
Vimentin also plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.