Thermo Fisher Scientific Dystrophin Polyclonal Antibody
Dystrophin 단백질을 검출하기 위한 Rabbit Polyclonal Antibody. Western blot, IHC 등 다양한 응용에 적합. Human과 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 기반 완충액에 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 3 publications |
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:400 | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human dystrophin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2277192 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, and porcine species based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is skeletal muscle tissue.
Target Information
Dystrophin is a 427 kDa protein encoded by the DMD/BMD gene located on the X chromosome (Xp21).
Western blotting and immunohistochemistry are established methods for detecting dystrophin expression abnormalities in muscle biopsies.
Dystrophin abnormalities are observed in nearly all patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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