Thermo Fisher Scientific Phospho-c-Raf (Ser338) Monoclonal Antibody (E.838.4)
Raf-1 단백질의 Ser338 인산화 부위를 인식하는 단일클론 항체로, 인간 및 설치류 시료에 반응합니다. Western blot 등에 적합하며, 액상 형태로 제공됩니다. -20°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunohistochemistry (IHC) | - | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| GST Pull Down (GPD) | - | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.838.4 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding serine 338 of human Raf |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 108 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10984169 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which subsequently activate ERK1 and ERK2. Activated ERKs regulate gene expression related to cell cycle, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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