Thermo Fisher Scientific Phospho-c-Raf (Ser338) Monoclonal Antibody (E.838.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

- -

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

GST Pull Down (GPD)

-

- -

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.838.4

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding serine 338 of human Raf if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Raf,uniProtId:P04049-1,ncbiNodeId:9606,antigenRange:338,antigenLength:648,antigenImageFileName:MA5-15176_c-Raf_P04049-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15176_c-Raf_P04049-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

108 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10984169

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.