Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Monoclonal Antibody (B.742.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:200

-

Product Specifications

Species Reactivity

Fruit fly, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

B.742.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERK1/2,uniProtId:P27361-1,ncbiNodeId:9606,antigenRange:202|204,antigenLength:379,antigenImageFileName:MA5-15174_ERK12_P27361-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15174_ERK12_P27361-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

98 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10980347

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.