Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr204) Monoclonal Antibody (B.742.5)
ERK1/ERK2의 Tyr204 인산화 상태를 검출하는 고특이성 단일클론 항체. Western blot, ICC/IF, Flow cytometry에 적합. 다양한 종에 반응하며 JNK/p38 MAPK와 교차 반응 없음. 액상 형태로 -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Human, Mink, Mouse, Non-human primate, Pig, Rat, Zebrafish |
| Published Species | Human, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B.742.5 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 98 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10980347 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric G protein-coupled receptors, and transforming agents, activate the ERK1 and ERK2 pathways.
When growth factors bind to receptor tyrosine kinases, Ras interacts with Raf, activating it. Raf phosphorylates MEK1/2, which in turn phosphorylates ERK1/2.
Activated ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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