Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Monoclonal Antibody (S.812.9)
Phospho-ERK1/ERK2 (Thr202, Tyr204) 단일클론 항체로 다양한 종에서 반응하며, WB, IHC, ICC, IP, ChIP 등 다중 응용 가능. ERK1/2 신호전달 경로 연구에 적합하며, 높은 특이성과 재현성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 5 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Immunoprecipitation (IP) | 1:100 | - |
| ChIP assay (ChIP) | 1–3 µL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Fruit fly, Hamster, Human, Mink, Mouse, Non-human primate, Pig, Rat, Yeast, Zebrafish |
| Published Species | Hamster, Human, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.812.9 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pThr202/Tyr204 of human p44 MAP kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 52.0 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_11009630 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase.
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many stimuli, including growth factors, cytokines, virus infection, ligands for G protein-coupled receptors, and transforming agents, activate the ERK1/2 pathways.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 항체의 항원 구조 이미지 포함)
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