Thermo Fisher Scientific ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA3-060 detects ADP-ribosylation factor 1 (Arf1), Arf3, Arf5, and Arf6, and about ten-fold less well with Arf4 from human, mouse, and rat tissues.
It has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures.
By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract.
Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes.
MA3-060 cannot be used to neutralize Arf in solution.
The MA3-060 antigen is recombinant human Arf1.

Target Information

ADP-ribosylation factor 1 (ARF1) belongs to the human ARF gene family, encoding small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D.
The ARF family includes 6 ARF proteins and 11 ARF-like proteins, part of the RAS superfamily.
ARF proteins are categorized as:

• Class I: ARF1, ARF2, ARF3
• Class II: ARF4, ARF5
• Class III: ARF6

Members of each class share a common gene organization.
ARF1 protein is localized to the Golgi apparatus and plays a central role in intra-Golgi transport.
Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
(Provided by RefSeq, Jul 2008)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.