Thermo Fisher Scientific Ki-67 Recombinant Rabbit Monoclonal Antibody (MKI67, 4947R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus through its forkhead associated (FHA) domain.
Ki-67 shows cell cycle-dependent topographical distribution: perinucleolar expression at G1, nuclear matrix localization at G2, and chromosomal association during M phase.
As a proliferation marker, Ki-67 is absent in G0 cells and increases steadily from G1 through mitosis. Ki-67 antibodies are useful for determining the cell growth fraction in neoplasms, with prognostic value comparable to the tritiated thymidine-labelling index. A low Ki-67 index correlates with histologically low-grade tumors. It is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.