Thermo Fisher Scientific Ki-67 Recombinant Rabbit Monoclonal Antibody (MKI67, 4947R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL
• Publications: -

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases.
It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle–dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
It serves as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis.
Ki-67 antibodies are widely used to assess proliferative activity in neoplasms, correlating with tumor grade and prognosis.
Additionally, Ki-67 is used as a neuronal marker for cell cycling and proliferation studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.