Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2466)
인간 Ki-67 단백질을 인식하는 마우스 단클론 항체로, 세포 증식 마커 분석에 사용됩니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 단백질 A/G 정제 및 4°C 보관이 가능합니다. 연구용으로만 사용됩니다.
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- 4288-MSM6-P0
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2466 |
| Immunogen | Recombinant fragment of human Ki67 protein (aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It associates with euchromatin, centromeric heterochromatin, and the nucleolus through its forkhead-associated (FHA) domain. Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosome-associated during mitosis.
Because Ki-67 is absent in G0 cells but increases steadily from G1 through mitosis, it serves as a reliable proliferation marker. Ki-67 antibodies are widely used to determine the growth fraction of neoplasms and have strong correlation with tumor grade. It is also a standard marker for neuronal cell cycling and proliferation studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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