Thermo Fisher Scientific EBI3 Polyclonal Antibody, Biotin
Mouse 및 Rat EBI3 단백질에 특이적인 Biotin 결합 폴리클로날 항체. Western blot, IHC, ELISA에 적합. 동결건조 형태로 제공되며 장기 보관 시 -20°C 권장. 인간 EBI3와 교차 반응 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant mouse EBI3 (repeated immunizations) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only before immediate use.
- Specific for mouse and rat EBI-3 protein.
- No cross-reactivity with human EBI-3.
- Cross-reactivity with IL-27 from other sources not determined.
Target Information
EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family involved in regulating Th2-type immune responses and Th2-mediated tissue inflammation. It forms heterodimers with IL-12 subunits (p28 and p35) to create IL-27 and IL-35, respectively.
EBI3 expression is regulated by TLR signaling via MyD88 and NF-κB in dendritic cells and influences Th cell development. It suppresses IL-17 and enhances IL-10 production, thereby modulating immune responses.
EBI3 may also play a role in controlling tumor metastasis through lung CD8+ T cells, and its deficiency is linked to reduced Th2 cytokine production and altered airway inflammation in asthma.
Targeted deletion of EBI3 protects mice from lung metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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