Thermo Fisher Scientific MIP-3 alpha Polyclonal Antibody, Biotin
마우스 및 랫트 MIP-3α(CCL20)에 반응하는 Biotin 결합 폴리클로날 항체. WB, IHC, ELISA에 적합하며 높은 민감도 제공. 단백질 A로 정제되어 높은 순도 보장. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3α protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids.
A control of similarly diluted normal rabbit IgG is recommended.
Target Information
Macrophage Inflammatory Protein-3 alpha (MIP-3α), also known as CCL20, is expressed in the liver, lungs, lymph nodes, and peripheral blood lymphocytes.
MIP-3α is strongly upregulated by inflammatory signals and downregulated by the anti-inflammatory cytokine IL-10.
It signals through the G protein-coupled receptor CCR6 and acts as a chemoattractant to lymphocytes and dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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