Thermo Fisher Scientific IL-27/p28 Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only immediately prior to use.

This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis suggests cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on ≥90% homology, there may be cross-reactivity with rat IL-27. Cross-reactivity with other species has not been determined.

Target Information

IL-27, a member of the IL-12 family, is a heterodimeric protein composed of the p40-related EBI3 subunit and the p28 (IL-30) subunit. It is produced by activated antigen-presenting cells and mature dendritic cells. IL-27 acts on NK cells and naive CD4+ T cells, and may also affect mast cells and monocytes through WSX-1/TCCR and gp130 receptor components.

Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 pathways, leading to STAT1/3 phosphorylation. WSX-1-deficient mice show impaired Th1 responses and increased susceptibility to Listeria monocytogenes infection, indicating IL-27’s essential role in initiating Th1 responses. IL-27 induces T-bet expression while suppressing GATA-3, promoting Th1 differentiation. It also exhibits antitumor activity by enhancing CTL responses and inhibiting tumor growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.