Thermo Fisher Scientific NAG-1 Polyclonal Antibody, Biotin
NAG-1 단백질을 인식하는 Biotin-conjugated Rabbit Polyclonal Antibody. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. Human 및 Mouse 반응성. Protein A 정제, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Immunogen
This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E. coli corresponding to full-length mouse NAG-1 protein. Cross-reactivity to MBP was removed via cross-adsorption chromatography.
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100% homology. Partial reactivity is expected against swine, bovine, and dog based on 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
Target Information
GDF-15 belongs to the TGF-beta cytokine family, which plays important roles during prenatal development, postnatal growth, and tissue remodeling. GDF-15 is predominantly expressed in the placenta and to a lesser extent in other tissues. Its presence in amniotic fluid and elevated levels in pregnant women suggest involvement in gestation and embryonic development. GDF-15 generally exhibits tumor-suppressive activities and is a major factor induced by the p53 pathway. Serum GDF-15 levels correlate positively with tumor progression in several cancers, including colorectal, pancreatic, and prostate cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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