Thermo Fisher Scientific HDAC9 Monoclonal Antibody (OTI2A7), TrueMAB

Applications and Tested Dilution

Product Specifications

Target Information

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation, and the acetylation of nucleosomal histones is a major mechanism of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in nucleosomal conformation and increased accessibility of DNA to transcription factors.

Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Conversely, deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1–9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, retain deacetylase activity, and repress myocyte enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.