Thermo Fisher Scientific c-Myc Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.5 µg/mL
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Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with regulation of proliferation and the cell cycle. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cell proliferation and differentiation, while deregulated expression can induce apoptosis. Antibodies against c-Myc epitopes recognize Myc-tagged proteins at either amino- or carboxy-termini. c-Myc, N-Myc, and L-Myc proteins are involved in neoplastic processes, and gene amplification has been observed in various human tumors including lung, breast, and colon carcinomas. Translocation of the c-myc locus is characteristic of Burkitt’s lymphoma and other B-cell malignancies. Aberrant c-myc expression is also found in tumors of diverse origins such as colorectal, gastric, hepatic, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.