Thermo Fisher Scientific Ki67 Polyclonal Antibody
Thermo Fisher Scientific의 Ki67 Polyclonal Antibody는 인간 Ki-67 단백질을 인식하는 토끼 유래 항체로, 세포 증식 마커로 널리 사용됩니다. Western blot, IHC, ICC/IF에 적합하며, 동결 건조 형태로 제공되어 안정적 보관이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human Ki67 recombinant protein (Position: K2860–I3256) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2884867 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that interacts with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle-dependent localization—perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
As a proliferation marker, Ki-67 is absent in G0 cells but increases from G1 through mitosis. It helps determine the growth fraction of neoplasms, with a strong correlation between low Ki-67 index and low-grade tumors. Ki-67 is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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