Thermo Fisher Scientific ErbB2 (HER-2) Polyclonal Antibody
ErbB2(HER-2) 단백질 검출용 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 합성 펩타이드 기반 면역원 사용. 연구용으로만 사용 가능.
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Thermo Fisher Scientific ErbB2 (HER-2) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:250 |
Product Specifications
| Item | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the region of human c-erbB2 containing non-phosphorylated tyrosine 1248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Gel ice packs |
| RRID | AB_2549814 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with the primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.
Target Information
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in certain breast tumors.
Herceptin, used in the treatment of metastatic HER2-positive cancer, is a monoclonal antibody targeting this kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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