Thermo Fisher Scientific CDK1 Polyclonal Antibody
CDK1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합합니다. 인간 CDC2 p34 C-말단 유래 합성 펩타이드로 면역화되었습니다. 다양한 종(소, 닭, 생쥐, 랫드) 반응 예측. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:25 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human CDC2 p34 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2549809 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, mouse, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is breast carcinoma or tonsil tissue.
Target Information
CDK1 (Cell Division Control protein 1) is essential for the completion of START, the controlling event in the cell cycle required to initiate mitosis.
CDK1 acts as the catalytic subunit of the M-Phase Promoting Factor, a protein kinase complex that induces entry into mitosis and is conserved among eukaryotes.
Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays apoptosis onset.
Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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