Thermo Fisher Scientific Granzyme B Monoclonal Antibody (GZMB, 3055)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature (RT).
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.

Target Information

Granzyme B is a member of the granzyme serine protease family, found in cytotoxic T cells and NK cells. It is crucial for the induction of apoptosis in target cells through caspase-3 activation and mitochondrial pathways. Granzyme B interacts with the mannose 6-phosphate receptor, functioning as a death receptor during cytotoxic T cell-induced apoptosis. It can cleave Bid protein to trigger mitochondrial apoptosis. Granzymes (A–G) are neutral serine proteases stored in lytic granules of CTLs and NK cells. In humans, fewer granzymes have been cloned compared to mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.