Thermo Fisher Scientific Granzyme B Monoclonal Antibody (GZMB, 3014)
Granzyme B 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 등에 사용 가능. 인간 시료에 반응하며, 비결합형 액상 형태로 제공. 단백질 A/G로 정제되었으며, 4°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GZMB, 3014 |
| Immunogen | Recombinant fragment of human GZMB protein (around aa 73–187) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Formalin-fixed tissues require heating in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Granzyme B is a member of the granzyme serine protease family found in cytotoxic T cells and NK cells. It plays a key role in inducing apoptosis of target cells by activating caspase-3 and initiating the caspase cascade. Granzyme B can also trigger mitochondrial apoptosis by cleaving Bid protein. It is known by alternative names such as CGL1 and CTLA-1, and functions via the mannose 6-phosphate receptor as a death receptor during cytotoxic T cell-induced apoptosis. Granzymes are neutral serine proteases stored in specialized lytic granules of cytotoxic lymphocytes and NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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