Thermo Fisher Scientific GCN4 Chimeric Recombinant Mouse Monoclonal Antibody (C11L34)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

10,000 ng/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Surface plasmon resonance (SPR)

Assay-dependent

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Mouse / IgG1, lambda

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

C11L34

Immunogen

Biotinylated GCN4-peptide (HLENEVARLKK). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCN4,uniProtId:P03069-1,ncbiNodeId:-23,antigenRange:266-276,antigenLength:281,antigenImageFileName:MA5-47957_GCN4_P03069-1_House_mouse.svg,antigenImageFileNamePDP:MA5-47957_GCN4_P03069-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074401

Product Specific Information

Specificity: This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity.

Target Information

GCN4 is a transcription factor protein in yeast that plays a crucial role in the regulation of genes involved in amino acid biosynthesis, such as HIS4, HIS7, HIS3, ARG1, ARG3, ARG4, and ARG5,6. The protein contains a basic leucine zipper (bZIP) domain that enables it to bind to specific DNA sequences, thereby regulating the transcription of target genes. The protein is also involved in the regulation of other cellular processes, including stress response and nutrient signaling, such as GSH1, YAP1, and TRX2. Its structure is composed of a dimeric coiled-coil domain, which allows for protein-protein interactions, and a DNA-binding bZIP domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.