Thermo Fisher Scientific Caspase 9 Monoclonal Antibody (LAP6 96-2-22)

Applications

Product Specifications

Product Specific Information

Suggested positive control: HEK 293 whole cell lysate.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, present in the cytosol, and upon activation translocates to the mitochondria.

Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Caspase 9 is expressed in various human tissues. An isoform of rat Caspase-9 has been identified with an alternative C-terminal sequence. Mutations in Caspase 9 affect brain development; insufficient Caspase 9 in mice results in abnormal cerebrum and neuron development. In humans, dysfunctional Caspase 9 expression varies among tissues. Low levels of Caspase 9 may contribute to cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.