Thermo Fisher Scientific GAPDH Monoclonal Antibody (1D4)
다양한 종에서 반응하는 GAPDH 단백질 검출용 단클론 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. 고순도 항원으로 제작되어 높은 특이성과 재현성을 제공하며, 단백질 로딩 컨트롤로 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 31 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100 | 2 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | 1:10 - 1:500 | - |
| GST Pull Down (GPD) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Bovine, Dog, Chicken, Fruit fly, Horse, Cat, Hamster, Human, Marsupial, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Zebrafish |
| Published Species | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D4 |
| Immunogen | Extensively purified pig GAPDH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.035% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_568547 |
Product Specific Information
This antibody is likely to react with most mammals.
Suggested positive control: antigen standard for GAPDH (transient overexpression lysate).
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH plays roles in:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
It is implicated in apoptosis, neurodegenerative diseases (e.g., Alzheimer’s and Huntington’s), and cancer-related processes. GAPDH interacts with proteins such as amyloid precursor protein and Huntingtin.
Due to its high expression across tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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