Thermo Fisher Scientific IkB alpha Recombinant Rabbit Monoclonal Antibody (116)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to prevent contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are generated using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• High specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broad immunoreactivity due to large rabbit immune repertoire

This antibody is specific for Human IkB alpha/NFKBIA.

Target Information

IkB-alpha is a 40 kDa protein that inhibits NF-kappaB activity through protein-protein interaction in the cytosol. The binding of IkB-alpha to NF-kappaB masks its nuclear localization sequence, preventing nuclear translocation.

Activation of NF-kappaB occurs when IkB-alpha is degraded in response to stimuli such as TNF-alpha, IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, and various stresses.

In humans, the IkB-alpha gene is located on chromosome 14q. Phosphorylation of IkB on serine residues 32 and 36 by IkB kinase alpha (IKKα, also known as CHUK) triggers its degradation. IKKα, together with IKKβ and IKKγ, forms the IKK complex essential for NF-kappaB activation. The NF-kappaB inducing kinase (NIK) regulates this process in response to TNF and IL-1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.