Thermo Fisher Scientific NBN Monoclonal Antibody (OTI2E10), TrueMAB
NBN 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, 동결건조 형태로 제공. Affinity chromatography로 정제되었으며, 재구성 후 약 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 183–460 of human NBN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The p95 gene (identical to NBS1 and nibrin) is part of the hMre11/hRad50 double-strand break repair complex.
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive disorder characterized by increased cancer susceptibility, cell cycle checkpoint defects, and ionizing radiation sensitivity.
This highlights the link between double-strand break repair mechanisms and cell cycle checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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