Thermo Fisher Scientific HA Tag Recombinant Mouse Monoclonal Antibody (2-2.2.14), Alexa Fluor Plus 594

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Biotin
• HRP
• Request custom conjugation

Product Specific Information

HA tag recombinant mouse monoclonal antibody specifically recognizes HA tag at both C and N-terminus of proteins.

Alexa Fluor™ Plus recombinant antibodies are conjugated using proprietary dye chemistry, providing brighter signal and improved signal-to-noise ratio compared to conventional Alexa Fluor™ conjugates. These recombinant antibodies exhibit high specificity and lot-to-lot consistency.

Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to minimize nonspecific background due to protein aggregates.

Applications Tested:
This 2-2.2.14 antibody has been tested by:

• Western blot at 4 µg/mL
• Immunocytochemistry at 5 µg/mL
• Flow cytometry ≤ 0.5 µg/test

A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Optimal antibody dilution should be empirically determined.

Excitation: 590 nm
Emission: 618 nm
Laser: Yellow Laser

Target Information

HA Tag (hemagglutinin tag) is an epitope tag used to localize and study proteins, identify associated complexes, and characterize low-abundance or poorly immunogenic proteins. Derived from influenza hemagglutinin, the HA tag facilitates detection and purification of tagged proteins for functional analysis. HA tag antibodies are commonly used for protein detection or co-immunoprecipitation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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