Thermo Fisher Scientific HA Tag Recombinant Mouse Monoclonal Antibody (2-2.2.14), Alexa Fluor Plus 555
HA 태그 단백질을 C 및 N 말단에서 특이적으로 인식하는 Alexa Fluor Plus 555 결합 항체. Western blot, ICC/IF, Flow Cytometry에 최적화. 높은 신호대잡음비와 일관된 로트 간 재현성 제공. 연구용 단백질 검출 및 정량 분석에 적합.
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Thermo Fisher Scientific HA Tag Recombinant Mouse Monoclonal Antibody (2-2.2.14), Alexa Fluor Plus 555
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 0.25–0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Expression System | ExpiCHO |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2-2.2.14 |
| Immunogen | HA peptide YPYDVPDYA derivitized to ovalbumin |
| Conjugate | Alexa Fluor™ Plus 555 |
| Excitation / Emission Max | 558 / 572 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | Proprietary buffer, pH 6.8 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- Unconjugated
- Alexa Fluor Plus 488
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Biotin
- HRP
- Custom conjugation available upon request
Product Specific Information
HA tag recombinant mouse monoclonal antibody specifically recognizes HA tag at both C and N-terminus of proteins.
Alexa Fluor™ Plus recombinant antibodies use proprietary dye chemistry for brighter fluorescence and improved signal-to-noise ratio compared to conventional Alexa Fluor conjugates.
These recombinant antibodies offer superior specificity and lot-to-lot consistency through gene cloning and controlled in vitro expression.
Usage Note:
Before use, centrifuge the conjugate solution briefly and use only the supernatant to minimize nonspecific background staining.
Applications Tested:
- Western blot: 2 µg/mL
- Immunocytochemistry: 2.5 µg/mL
- Flow cytometry: ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Titrate carefully for optimal performance.
Optical Properties:
- Excitation: 553 nm
- Emission: 568 nm
- Recommended Laser: Yellow Laser
Target Information
HA Tag (hemagglutinin tag) is an epitope tag used to localize gene products, study protein topology, identify associated proteins, and characterize low-abundance or poorly immunogenic proteins.
Derived from influenza hemagglutinin, HA tag facilitates protein detection and purification for functional analysis.
HA tag antibodies enable reliable detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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